Figure 5. Effect of mild hypothermic growth conditions on the response of CHO cells to shear.

Samples taken from fed-batch cultures operated at 32 or 37°C were exposed to shear environments that represent those typically experienced in industrial scale centrifuges. The viability determined prior to and after application of the shear using a Vi-Cell XR. The plots depicted here show the results from samples that had the same pre#x02010;shear viability, represented here as high [37°C, day 10 (•); 32°C, day 12 (▲)] and low [37°C, day 12 (○); 32°C, day 20 (Δ)]. n = 3 (repeat viability measurements) ± s.d.