Figure 6. Effect of mild hypothermic culture conditions on centrifugation performance predicted using an USD model.

Samples taken from fed-batch cultures operated at 32 or 37°C were exposed to shear environments that represent those typically experienced in industrial scale centrifuges. The predicted clarification performance was determined by centrifugation of sheared and non-sheared samples in 96-well plates conditions to give V/c.t.Σ values of 1.88 (•,○), 2.56 (▲,Δ) and 5.00 × 10⁻⁸ m s⁻¹ (▪,□), 32°C (filled symbols), 37°C (empty symbols).