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. 2013 Apr 24;43(6):1470–1483. doi: 10.1002/eji.201242935

Figure 3.

Figure 3

Uptake of HIV-1 involving β7-integrins on DCs significantly decreased both MHCI and MHCII presentation. (A–D) IDCs and MDCs were preincubated with anti-β7 mAb (20 μg/mL) followed by overnight incubation with free HIV-1BaL (F-HIV) or complement opsonized HIV-1BaL (C-HIV). The different groups of DCs were incubated overnight, washed, and cocultured with (A and B) an HIV-1 gag p17 SL9 (SLYNTVATL) specific CD8+ T-cell clone or (C and D) an HIV-1 p24 LI13 (LNKIVRMYSPTS) specific CD4+ T-cell clone. The T-cell responses were assessed by IFN-γ ELISPOT assay. Data are normalized with F-HIV as 100% and shown as mean +SEM of 4 to 8 independent experiments. *p<0.05, **p<0.005, two-sided paired t-test.