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. 2013 Apr 24;43(6):1470–1483. doi: 10.1002/eji.201242935

Figure 7.

Figure 7

F-HIV and C-HIV modulated factors involved in transport and transport kinetics of protein targeted for proteasome degradation in IDCs and MDCs. (A–J) IDCs and MDCs were incubated with free HIV-1BaL (F-HIV) or complement opsonized HIV-1BaL (C-HIV) (75 ng p24CA equivalent/group) for 48 h. After the incubation, the different groups of DCs were harvested, mRNA was extracted, and gene expression levels of (A and B) ISG15, (C and D) USP18, (E and F) UBE2L6, (G and H) NEDD8, and (I and J) PSME2 were assessed by qRT-PCR. Data are normalized with mock DCs as 100% and are shown as mean +SEM of six independent experiments. *p<0.05, two-sided paired t-test.