Table 1.
Expression of ubiquitin-like pathways in IDCs and MDCs after exposure to F-HIV or C-HIVa
| iDCsb | ||
|---|---|---|
| Pathway (number of proteins identified) | F-HIV | C-HIV |
| Ubiquitination, E1 proteins (2) | 1.02 (± 0.07) | 0.97 (± 0.07) |
| Ubiquitination, E2 proteins (8) | 0.89 (± 0.10) | 0.95 (± 0.05) |
| Ubiquitination, E3 proteins (14) | 1.04 (± 0.08) | 0.97 (± 0.05) |
| Deubiquitination (11) | 1.03 (± 0.05) | 1.01 (± 0.04) |
| SUMO pathway (7) | 1.04 (± 0.05) | 0.98 (± 0.04) |
| NEDD8 pathway (5) | 1.76 (± 0.63) | 1.33 (± 0.36) |
| ISG15 pathway (4) | 5.54 (± 4.08) | 3.38 (± 2.56) |
| iDCs | ||
| NEDD8 pathway proteins | F-HIV | C-HIV |
| NEDD8 | 0.80 (± 0.00) | 0.8 (± 0.00) |
| UBC12 (NEDD8-conjugating enzyme Ubc12) | 0.95 (± 0.05) | 0.95 (± 0.05) |
| UBA3 (NEDD8-activating enzyme E1 catalytic subunit) | 1.05 (± 0.15) | 0.95 (± 0.05) |
| NAE1 (NEDD8-activating enzyme E1 regulatory subunit) | 0.80 (± 0.00) | 0.75 (± 0.05) |
| NUB1 (NEDD8 ultimate buster 1) | 3.73 (± 0.53) | 2.4 (± 0.46) |
| iDCs | ||
| ISG15 pathway proteins | F-HIV | C-HIV |
| ISG15 (ubiquitin-like protein ISG15) | 15.87 (± 7.43) | 8.9 (± 5.81) |
| UBA7 (ubiquitin-like modifier-activating enzyme 7) | 1.35 (± 0.35) | 1.05 (± 0.05) |
| UBE2L6 (ubiquitin/ISG-conjugating enzyme E2 L6) | 2.1 (± 0.50) | 1.5 (± 0.37) |
| TRIM25 (E3 ubiquitin/ISG15 ligase TRIM25) | 1.43 (± 0.16) | 1.3 (± 0.14) |
| mDCsc | ||
| Pathway (number of proteins identified) | F-HIV | C-HIV |
| Ubiquitination, E1 proteins (2) | 0.99 (± 0.10) | 0.96 (± 0.06) |
| Ubiquitination, E2 proteins (11) | 0.89 (± 0.08) | 0.89 (± 0.07) |
| Ubiquitination, E3 proteins (17) | 0.90 (± 0.04) | 0.94 (± 0.09) |
| Deubiquitination (19) | 0.96 (± 0.04) | 0.95 (± 0.04) |
| SUMO pathway (8) | 1.20 (± 0.12) | 1.12 (± 0.08) |
| NEDD8 pathway (5) | 1.00 (± 0.05) | 1.06 (± 0.07) |
| ISG15 pathway (4) | 0.96 (± 0.07) | 0.99 (± 0.07) |
a)
Expression of proteins in the indicated pathways was determined by quantitative MS proteomics 24 h after exposure of IDCs or MDCs to F-HIV or C-HIV.
b)
Data are normalized with mock-treated IDCs as 1 and shown as mean ± SEM of three independent experiments.
c)
Data are normalized with mock-treated MDCs as 1 and shown as mean ± SEM of four independent experiments.