Table 2.
Mapping results of test data onto human genome build hg19
Sample | Total reads | Uniquely mapped reads | % | Reads mapped to multiple loci* | % | Unmapped reads | % | Reads filtered as PCR bias | % | Reads remaining after filtering | % |
---|---|---|---|---|---|---|---|---|---|---|---|
Rad21 (HeLa) | 76 354 774 | 49 710 285 | 65.10 | 7 472 860 | 9.79 | 19 171 629 | 25.11 | 5 329 568 | 6.98 | 44 380 717 | 58.12 |
Smc3ac (HeLa) | 121 365 117 | 76 858 790 | 63.33 | 11 892 522 | 9.80 | 32 613 805 | 26.87 | 15 364 647 | 12.66 | 61 494 143 | 50.67 |
CTCF (HeLa) | 42 742 350 | 28 754 522 | 67.27 | 3 736 358 | 8.74 | 10 251 470 | 23.98 | 4 359 289 | 10.20 | 24 395 233 | 57.08 |
Control (HeLa) | 111 252 274 | 67 341 466 | 60.53 | 9 881 093 | 8.88 | 3 402 971 | 3.06 | 5 822 912 | 5.23 | 61 518 554 | 55.30 |
When using multiple mapped reads, the number of usable reads increases by approximately 10%.