Figure 2.

Stable egfp expression in ubi-egfp vector-transduced Crandall-Rees feline kidney (CRFK) cells. Stability of transgene expression is expressed as the percentage of Egfp‑positive cells (top panel) and the mean fluorescence intensity (MFI, bottom panel). CRFK cells were transduced with pCF-SIN-ubi-egfp and pCF-7-ubi-egfp at a multiplicity of infection (MOI) of 0.5. Transduced cells were passaged twice weekly and analyzed by flow cytometry every three weeks post-transduction. Quantitative results are given by the percentage of Egfp-positive cells and the MFI as a marker for the expression level, according to each flow cytometry assay.