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. 2013 Jul 17;5(7):2734–2746. doi: 10.3390/nu5072734

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Histological images of liver tissues from OVX-induced hepatic steatosis displaying the hepatoprotective effects of PTIF. There were no histological abnormalities observed in sham rats (A). OVX-induced hepatic steatosis showing hepatocytes with severe cytoplasmic vacuoles and swelling (B). E2 10 μg/kg resulted in the prevention of hepatic fatty deposition in hepatocytes (C). The treatments with PTIF 30 and 100 mg/kg resulted in the prevention of hepatic fatty deposition in hepatocytes (D,E). The tissues were surgically excised and subjected to histological study by staining with hematoxylin and eosin (H & E). All magnifications: 200×. The arrows indicate fatty hepatocytes.