Figure 6.

Histone acetylation score predicts for sensitivity of HMCLs to panobinostat, valproic acid (VPA) or SAHA HDACi. Human myeloma cell lines with a high HA Score (N=5) exhibit significant higher sensitivity to panobinostat, VPA or SAHA compared with HMCLs with a low HA Score (N=5). Human myeloma cell lines were cultured for 4 days in 96-well flat-bottom microtitre plates in RPMI 1640 medium, 10% fetal calf serum, 2 ng ml⁻¹ IL-6 culture medium (control) and graded concentration of panobinostat (A), VPA (B) or SAHA (vorinostat) (C). Data are mean values± standard deviation (s.d.) of five experiments determined on sextuplet culture wells.