Table 1.
Primers for real-time RT-PCR
| Name | Sequences | Annealing temperature | Product size (bp) |
|---|---|---|---|
| TLR1 | F:5'-CTATACACCAAGTTGTCAGC-3' | 60 | 220 |
| R:5'-GTCTCCAACTCAGTAAGGTG-3' | |||
| TLR2 | F:5'-GCCAAAGTCTTGATTGATTGG-3' | 64 | 347 |
| R:5'-TTGAAGTTCTCCAGCTCCTG-3' | |||
| TLR4 | F:5'-TGGATACGTTTCCTTATAAG-3' | 56 | 507 |
| R:5'-GAAATGGAGGCACCCCTTC-3' | |||
| TLR5 | F:5'-CTAGCTCCTAATCCTGATG-3' | 56 | 438 |
| R:5'-CCATGTGAAGTCTTTGCTGC-3' | |||
| TLR6 | F:5'-CCTCCCAGGATCAAGGTACTTG-3' | 60 | 327 |
| R:5'-ATCAGGCCAGCCCTCTAACAC-3' | |||
| TLR9 | F:5'-CCCTCAACTTCACCTTGGATCT-3' | 64 | 408 |
| R:5'-CCACATATGGCCCAGTGCA-3' | |||
| IL-1β | F:5'-TGATGGCTTATTACAGTGGCAATG-3' | 140 | 60 |
| R:5'-GTAGTGGTGGTCGGAGATTCG-3' | |||
| IL-6 | F:5'-GTGTTGCCTGCTGCCTTC-3' | 60 | 194 |
| R:5'-AGTGCCTCTTTGCTGCTTTC-3' | |||
| IL-8 | F:5'-GACATACTCCAAACCTTTCCAC-3' | 60 | 160 |
| R:5'-CTTCTCCACAACCCTCTGC-3' | |||
| IL-10 | F:5'-GAACCAAGACCCAGACATC-3' | 60 | 137 |
| R:5'-CATTCTTCACCTGCTCCAC-3' | |||
| IL-12p40 | F:5'-TCGGCAGGTGGAGGTCAGC-3' | 60 | 77 |
| R:5'-CGCAGAATGTCAGGGAGAAGTAGG-3' | |||
| IFN-γ | F:5'-TGTGGAGACCATCAAGGAAGAC-3' | 60 | 121 |
| R:5'-TGCTTTGCGTTGGACATTCAAG-3' | |||
| TNF-α | F:5'-ATCTTCTCGAACCCCGAGTG-3' | 60 | 51 |
| R:5'-GGGTTTGCTACAACATGGGC-3' | |||
| iNOS | F:5'-TGGATGCAACCCCATTGTC-3' | 60 | 59 |
| R:5'-CCCGCTGCCCCAGTTT-3' | |||
| eNOS | F:5'-CGGCATCACCAGGAAGAAGA-3' | 67 | 60 |
| R:5'-CATGAGCGAGGCGGAGAT-3' | |||
| β-actin | F:5'-GCGAGAAGATGACCCAGATC-3' | 60 | 77 |
| R:5'-GGATAGCACAGCCTGGATAG-3' |
RT-PCR: real time-polymerase chain reaction; TLR: Toll-like receptor; NOD: nucleotide-binding oligomerization domain.