Table 1.
Antibodies used for immunohistochemistry and immunofluorescence
| Marker | Antibody | Recognition status | Host Species | Dilution | Vendor |
|---|---|---|---|---|---|
| TAU | |||||
| S202/T205 | AT8 | PHF tau - hyperphosphorylated | mouse | 1:10 000 | ThermoScientific |
| S199/202 | Ser199/202 | PHF tau - hyperphosphorylated | rabbit | 1:100 000 | Anaspec |
| S396 | Ser396 | PHF tau - hyperphosphorylated | rabbit | 1:30 000 | Anaspec |
| S356 | Ser356 | PHF tau - hyperphosphorylated | rabbit | 1:10 000 | Anaspec |
| total tau | H-150 | rabbit | 1: 3 000 | SantaCruz Biotechnology | |
|
| |||||
| Microglia/Astrocyte | |||||
| CD45 | activated microglial | rat | 1:30 00 | Serotec | |
| CD11B | general microglia | rat | 1:1 000 | Serotec | |
| CD68 | phagocytic microglia | rabbit | 1:3 000 | Serotec | |
| GFAP | activated astrocyte | rabbit | 1:10 000 | Dako | |
|
| |||||
| Anyloid beta | |||||
| polyclonal Aβ | Aβ 40/42 | rabbit | 1:10 000 | Gift from Dr. P. Gottschall | |
|
| |||||
| Kinase | |||||
| GSK3 α/β pTyr 216+279 |
GSK3 | activated GSK α/β | rabbit | 1:3 000 | Abcam |
Abbreviations: CD, cluster of differentiation; GFAP, glial fibrillary acidic protein; GSK 3, glycogen synthase kinase; S, serine; T, threonine; Tyr, tyrosine; p, phosphorylated.