Figure 4.
a) 6 M Gn•HCl, 100 mM Na2HPO4, 50 mM TCEP, pH 7.2. b) 6 M Gn•HCl, 300 mM Na2HPO4, 200 mM MPAA, 20 mM TCEP, pH 7.2. Gn = guanidine, TCEP = tris(2-carboxyethyl)phosphine hydrochloride, MPAA = 4-mercaptophenylacetic acid.
a) 6 M Gn•HCl, 100 mM Na2HPO4, 50 mM TCEP, pH 7.2. b) 6 M Gn•HCl, 300 mM Na2HPO4, 200 mM MPAA, 20 mM TCEP, pH 7.2. Gn = guanidine, TCEP = tris(2-carboxyethyl)phosphine hydrochloride, MPAA = 4-mercaptophenylacetic acid.