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. Author manuscript; available in PMC: 2013 Aug 9.
Published in final edited form as: J Proteome Res. 2012 Dec 13;12(1):316–327. doi: 10.1021/pr300408v

Figure 2.

Figure 2

(A) Schematic illustration of Cdc13 domain organization. (B) Phosphatase treatment of Cdc13. 1.5 µg of purified Cdc13 was incubated with 40 U of λ protein phosphatase (NEB, lanes 2 and 3) and 1 mM MnCl2 (lanes 3 and 4) in 1X vendor-supplied buffer at 30 °C for 30 min. Samples were resolved on 8% SDS-PAGE gel and stained with Coomassie blue.