Telomere length analysis of Cdc13 phosphorylation mutants. cdc13Δ cells carrying WT or mutant Cdc13 on a CEN TRP1 plasmid were analyzed for their telomere lengths as described in Methods. Mutants analyzed are (A) S314A, S314D, S324A, S324D, S314A S324A, and S314D S324D, and (B) cdc13-2 and cdc13-2 S314A mutants. (C) Western blot analysis of myc9-tagged Cdc13 mutants expressed from Cdc13 promoter on a CEN TRP1 plasmid (upper panel). The same blot was probed with anti-α tubulin as loading control (lower panel). UT: untagged.