Table 1.
position | flanking sequence | telomere length | G1/G2 abundance | WT/MT abundance | putative kinase |
---|---|---|---|---|---|
S56 | CLLGFSNFER | ND | ND | ND | |
S225 | QKVLSQKSK | unchangedb | ND | ND | Tel1/Mec1b |
S306 | KSYIQSQTPE | unchanged | unchanged | unchanged | Mec1c |
T308 | KSYIQSQTPE | T308A: slightly shortc | moderate increase in G2/M |
unchanged | CDK1c |
S314 | RKTSVPNNW | S314A: slightly longer in cdc13-2 background |
substantial increase in G2/M |
drastic increase in WT in G2/M | |
S314D: slightly short | |||||
S324 | DDDSGSKR | S324A, S324D: unchanged | substantial increase in G2/M |
drastic increase in WT in G2/M | |
S333 | KLSFHSPNAS | ND | unchanged | drastic increase in WT in G1 | |
S336 | KLSFHSPNAS | S336A: unchangedc | unchanged | drastic increase in WT in G1 | CDK1c |
S340 | FHSPNASSIR | ND | ND | ND | |
S341 | SSIRKAISY | ND | moderate increase in G2/M |
unchanged | |
S347 | SSIRKAISY | ND | unchanged | moderate decrease in WT | |
S352 | LSLASVGSVE | ND | ND | ND | |
S460 | DKTASPGMA | ND | unchanged | unchanged | CDK/MAPK (not CDK1)c |
S708 | VKHEPKKSPT | S708A: slightly longer | moderate increase in G1 |
unchanged | CDK/MAPK (not CDK1)c |
S710 | SPTTPALAE | ND | unchanged | unchanged | |
S711 | SPTTPALAE | T711A: unchanged | unchanged | unchanged | CDK/MAPK (not CDK1c) |
S726 | LNADVSSFD | ND | ND | ND | |
S727 | LNADVSSFD | ND | ND | ND | |
S883 | PDSGSLDCAI | ND | unchanged | moderate decrease in WT in G1 but moderate increase in WT in G2/M |
|
S885 | PDSGSLDCAI | ND | unchanged | unchanged | |
T893 | NATVNPLRLL | T893A, T893D: unchanged | ND | ND |
The table indicates the position of phosphorylation, effect on telomere lengths, and change of abundance between cell cycle phases, and that between WT Cdc13 and the Cdc13-E252K mutant (MT). Sites from different domains are separated by dashed lines. The change in phosphorylation abundance for each site was derived from the most consistently observed peptide. Moderate, substantial, and drastic reflect a 2–5-fold, 5–10-fold, and more than 10-fold change in abundance. ND: not determined.
Result reported in a previously published work.36