Table 1.

Summary of Cdc13 Phosphorylation Sites Identified from Full-Length, WT Protein^a

position	flanking sequence	telomere length	G1/G2 abundance	WT/MT abundance	putative kinase
S56	CLLGFSNFER	ND	ND	ND
S225	QKVLSQKSK	unchangedb	ND	ND	Tel1/Mec1b
S306	KSYIQSQTPE	unchanged	unchanged	unchanged	Mec1c
T308	KSYIQSQTPE	T308A: slightly shortc	moderate increase in G2/M	unchanged	CDK1c
S314	RKTSVPNNW	S314A: slightly longer in cdc13-2 background	substantial increase in G2/M	drastic increase in WT in G2/M
		S314D: slightly short
S324	DDDSGSKR	S324A, S324D: unchanged	substantial increase in G2/M	drastic increase in WT in G2/M
S333	KLSFHSPNAS	ND	unchanged	drastic increase in WT in G1
S336	KLSFHSPNAS	S336A: unchangedc	unchanged	drastic increase in WT in G1	CDK1c
S340	FHSPNASSIR	ND	ND	ND
S341	SSIRKAISY	ND	moderate increase in G2/M	unchanged
S347	SSIRKAISY	ND	unchanged	moderate decrease in WT
S352	LSLASVGSVE	ND	ND	ND
S460	DKTASPGMA	ND	unchanged	unchanged	CDK/MAPK (not CDK1)c
S708	VKHEPKKSPT	S708A: slightly longer	moderate increase in G1	unchanged	CDK/MAPK (not CDK1)c
S710	SPTTPALAE	ND	unchanged	unchanged
S711	SPTTPALAE	T711A: unchanged	unchanged	unchanged	CDK/MAPK (not CDK1c)
S726	LNADVSSFD	ND	ND	ND
S727	LNADVSSFD	ND	ND	ND
S883	PDSGSLDCAI	ND	unchanged	moderate decrease in WT in G1 but moderate increase in WT in G2/M
S885	PDSGSLDCAI	ND	unchanged	unchanged
T893	NATVNPLRLL	T893A, T893D: unchanged	ND	ND

^aThe table indicates the position of phosphorylation, effect on telomere lengths, and change of abundance between cell cycle phases, and that between WT Cdc13 and the Cdc13-E252K mutant (MT). Sites from different domains are separated by dashed lines. The change in phosphorylation abundance for each site was derived from the most consistently observed peptide. Moderate, substantial, and drastic reflect a 2–5-fold, 5–10-fold, and more than 10-fold change in abundance. ND: not determined.

^bResult reported in a previously published work.³⁶

^cResults reported in previously published works.^37,38