Figure 3.
Expression of StAR mRNA in mouse Leydig cells is inhibited by mibefradil. Acutely purified mouse Leydig cells were incubated in 1.0 mmol L−1 Ca2+-containing DMEM/F12 medium and stimulated by 100 IU hCG treatment in the presence of mibefradil at 0.01, 0.1 or 1.0 mmol L−1 for 1 h before StAR mRNAs were semi-quantitatively amplified in RT-PCR. (A) A representative RT-PCR profile of StAR mRNA expression. (B): The band intensities of StAR mRNA products were quantified densitometrically and expressed relative to control (that is, no mibefradil). Data are means ± SD of results from five independent experiments. *P < 0.05 compared with control (100%).