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. 2010 Jan 25;12(2):186–195. doi: 10.1038/aja.2009.76

Figure 5.

Figure 5

Effects of transgelin on intrinsic apoptotic pathway-related proteins. (A): Effect of transgelin on apoptosis-related genes in LNCaP cells. LNCaP cells were transfected with vector or transgelin followed by incubation for the indicated time periods, and cell lysate was analyzed for Western blotting to detect the expression of Puma, Bcl-2, Bax and caspase-3. GAPDH was used as internal control. (B): Function of transgelin depended on wild-type p53. HCT116 and HCT116-p53−/− cells were transfected with pcDNA3.1-transgelin or vector, and followed by harvest after 24 h. The cell lysate was analyzed by western blotting to evaluate p53 and Puma expression.