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. 2011 Jan 17;13(2):266–274. doi: 10.1038/aja.2010.149

Figure 3.

Figure 3

2D silver stain gels of human sperm extracts immunoprecipitated by rat anti-CABYR-A polyclonal antibody (a) and pre-immune serum (b). Three potential positive spots were removed from gels and microsequenced by mass spectrometry. Spot 1 is inter-alpha-inhibitor H4 heavy chain [Rattus norvegicus] (accession no. AAH89806.1); it should come from the rat antiserum; spot 2 contains inter-alpha-inhibitor H4 heavy chain [Rattus norvegicus] and HSP90AA1 protein (accession no. AAH23006.2) sequences; Spot 3 is human Ropporin (accession no. AAG27712.2.). The CABYR-A spot is indicated by the upper arrow. CABYR, calcium-binding tyrosine phosphorylation-regulated protein; 2D, two-dimensional; HSP90, heat-shock protein 90; MW, molecular weight; pI, isoelectric point.