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. 2010 Dec 20;13(2):254–265. doi: 10.1038/aja.2010.99

Figure 4.

Figure 4

Western blots for the various GLAST epitopes studied. Lane 1 was from brain; lanes 2–5 were testis, labeled for N-terminal GLAST (lane 1, 2), C-terminal GLAST (lane 3), exon-3-skipping GLAST1a (lane 4) and exon-9-skipping GLAST1b (lane 5). N-terminal GLAST labeling was present in the brain (running as a broad monomer band of 50–60 kDa and as a broad dimer band at around 100–150 kDa, but was absent in the testis). C-terminal GLAST labeling was present in the testis (∼55 kDa); GLAST1a was abundant and ran at the expected size (∼55 kDa) and as an ∼110-kDa band, which is thought to represent GLAST1a dimers. A single band of about 55–60 kDa was detected using GLAST1b antibodies. GLAST, glutamate aspartate transporter.