Immunolabeling for various forms of GLAST in the testis. Immunostaining for N-terminal GLAST was absent (a), whereas labeling for C-terminal GLAST (b, c) was present in interstitial cells (I). Labeling for GLAST1b (d) was similarly present in interstitial cells, while labeling for GLAST1a (e, f) was present both in interstitial cells and in a subset of seminiferous tubules (T), where it appeared to be associated with the heads of sperm (arrowheads). Scale bars: a
b, d, e=200 µm; c, f=100 µm. GLAST, glutamate aspartate transporter.