Table 1. Primers used for identification of EAATs in the testis.
| EAAT member | GenBank accession | Forward oligo sequence | Reverse oligo sequence | Product size (bp) |
|---|---|---|---|---|
| GLAST | NM_019225.1 | TGACAAAAAGCAACGGAGAAGAGC | TATCAGAGTAGGGAGGAAAGAGGA | 1743 |
| GLAST1a | AF265360 | CATTGTGGGAATGGCGGC | TATCAGAGTAGGGAGGAAAGAGGA | 1432 |
| GLAST1b | AY954110 | ATGGAGACTCTGACCCGGATC | GGAGGCGGTCCCTTATTGTTAT | 629 |
| GLT1a | X67857.1 | GCTCGTCGCCACTGTCTCCA | CATAAGATACGCTGGGGAGTT | 1824 |
| GLT1b | AF465909.1 | ACTTTGCCTGTCACCTTCCGTTGC | ATGCAGGTCTCGATATCCAGG | 594 |
| GLT1c | AY578981.1 | ACTTTGCCTGTCACCTTCCGTTGC | TAAACCCACGATTGATATTCCACA | 591 |
| EAAC1 | NM_013032.3 | CGCTCCCCGATTCCTCACAAA | CTTCACAGTCCCAGGCATCTA | 1638 |
| EAAT4 | NM_032065.1 | CGCTGACCCGAGGCTGAG | CCGCCCTGGACAAAGAGCTA | 1736 |
| EAAT5 | NM_001108973.1 | ACAACCTACAGTGCATGGAAT | ACTCCTACGCTTAGCCCAAAC | 1763 |
Abbreviations: EAAC, excitatory amino acid carrier; EAAT, excitatory amino acid transporter; GLAST, glutamate aspartate transporter; GLT, glutamate transporter.