Figure 1.

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of affinity-purified gelatin-binding proteins from human seminal plasma. Pooled human seminal plasma from subjects with normal semen parameters was applied to gelatin–Sepharose 4B column, and the bound fraction was eluted with 4 mol L⁻¹ urea. The isolated preparation was subjected to SDS-PAGE on a 4%–20% gradient gel under reducing conditions. The proteins were stained with silver. The numbers indicate molecular mass (kDa) of standard proteins used for gel calibration. The electrophoretic pattern is indicated by arrowheads.