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. 2010 Feb 22;12(3):363–375. doi: 10.1038/aja.2009.98

Figure 2.

Figure 2

Normal phase (NP20) protein chip profiling of affinity-purified gelatin-binding proteins from human seminal plasma. Affinity-purified gelatin-binding proteins from human seminal plasma were applied to NP20 ProteinChip array and processed as indicated in Materials and methods. A surface-enhanced laser desorption/ionization-time of flight (SELDI-TOF) mass spectrum was acquired in ion-positive mode at 25 kV and analyzed using CiphergenExpress Software 3.0 (BioRad, Hercules, CA, USA). Calibration was performed with ProteinChip all-in-one protein standards II. Molecular mass ranges are as follows: (A) 8–17 kDa, (B) 20–50 kDa, (C) 50–90 kDa and (D) 90–250 kDa.