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. 2010 Feb 22;12(3):363–375. doi: 10.1038/aja.2009.98

Figure 4.

Figure 4

Heparin-binding pattern of affinity-purified gelatin-binding proteins from human seminal plasma. Heparin (spectrum A) or gelatin (spectrum B) were immobilized on preactivated surface (PS20) ProteinChip array and allowed to react with affinity-purified gelatin-binding proteins, as described in Materials and methods. On-chip ligand-captured molecules were detected by SELDI-TOF mass spectrometry in ion-positive mode at 25 kV and analyzed using CiphergenExpress Software 3.0 (BioRad, Hercules, CA, USA). Calibration was performed with ProteinChip all-in-one protein standards II.