Figure 6.

Metal-binding properties of affinity-purified gelatin-binding proteins from human seminal plasma. IMAC30 ProteinChip array charged with Fe³⁺ ions was prepared and incubated with affinity-purified gelatin-binding proteins, as indicated in Materials and methods. The bound proteins were detected by SELDI-TOF mass spectrometry in ion-positive mode at 25 kV and analyzed using CiphergenExpress Software 3.0 (BioRad, Hercules, CA, USA). Calibration was performed with ProteinChip all-in-one protein standards II.