Skip to main content
. Author manuscript; available in PMC: 2013 Aug 9.
Published in final edited form as: Virology. 2008 Sep 18;380(2):285–295. doi: 10.1016/j.virol.2008.07.007

Fig. 1.

Fig. 1

Effect of sCD4 on neutralization of JRFL, ADA and YU2 by anti-V3 mAbs 447-52D, 2219, 3074 and anti-co-receptor mAb 17b. (A) Viral entry expressed as luciferase (RLU) of JRFL is shown with varying concentrations of sCD4. Black bar shows viral entry with no mAb and no sCD4 present. Grey bar shows viral entry with 447-52D. White bars show the effect of sCD4 alone on viral entry. Hatched bars show the effect of each concentration of sCD4 with a fixed amount (10 ug/ml) of mAb 447-52D. (B) The percent adjusted neutralization was calculated from (A) using RLU values for each concentration of sCD4 as the baseline for viral entry. The additional effect on viral entry by 10 ug/ml of mAb 447-52D is the percent adjusted neutralization. (C), (D) and (E) show the percent adjusted neutralization by several mAbs against viruses JRFL, ADA and YU2. Legend in (C) applies to (D) and (E). (F) Serial dilutions of mAb 447-52D alone, or with a fixed concentration of sCD4 were tested to calculate the impact of sCD4 on the potency of mAb 447-52D neutralization. Note that for graphs C, D and E, the IC50 and IC80 effect of sCD4 against each virus are indicated on the X-axis.