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. Author manuscript; available in PMC: 2014 Jan 18.
Published in final edited form as: Nature. 2013 Jul 18;499(7458):301–305. doi: 10.1038/nature12343

Figure 2. Myomaker is essential for skeletal muscle development.

Figure 2

a, Full term WT (+/+) and Myomaker−/− embryos were dissected and skinned to illustrate the lack of muscle surrounding Myomaker−/− limbs. b, Paraffin sectioning and H&E staining on tongues reveal a lack of muscle fibers in E17.5 Myomaker−/− embryos. c, Longitudinal sections of E14 hindlimb muscles stained with a myosin antibody to determine the multi-nucleation of the muscle cells. WT limbs exhibit myofibers containing multiple nuclei, which are absent in Myomaker−/− sections. Scale bars: a, 2 mm; b, 100 µm; c, 40 µm.