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. Author manuscript; available in PMC: 2014 Jan 18.
Published in final edited form as: Nature. 2013 Jul 18;499(7458):301–305. doi: 10.1038/nature12343

Figure 3. Control of myoblast fusion by Myomaker.

Figure 3

a, Myoblasts from WT (+/+) and Myomaker−/− E17 embryos were differentiated for 3 days, and stained for myosin and a nuclear stain (Hoechst). Myomaker−/− myoblasts failed to fuse. b, Quantitation of the number of nuclei present in a myosin+ cell indicates Myomaker−/− myoblasts cannot form myotubes with three or more nuclei. c, Differentiation index, calculated as the percentage of nuclei in myosin+ cells, indicated null myoblasts can activate the myogenic program. d, C2C12 cells infected with a retrovirus encoding GFP or Myomaker were induced to differentiate for 4 days then stained with a myosin antibody and Hoechst (nuclei). e, Quantitation of the percentage of myosin+ cells that contained the indicated number of nuclei. Quantification was performed after 3 days of differentiation in (b), (c), and after 4 days in (e). Scale bars: a, 100 µm e, 200 µm. Data are presented as mean ± SEM from three independent experiments. * P < 0.05 compared to +/+ in b, c or GFP-infected cells in e. ns in c is not statistically significant.