Skip to main content
. 2010 Jun 7;12(5):709–717. doi: 10.1038/aja.2010.39

Figure 1.

Figure 1

Knockdown of the nucleosome-binding protein 1 (NSBP1) expression by siRNA in vitro and in vivo. Lentivirus short hairpin constructs against NSBP1 (PscSI616) were transfected into DU145 cells. (A): NSBP1 mRNA relative to GAPDH was examined 5 days after infection by real-time quantitative RT-PCR in comparison with PscNC control and blank DU145 cells. The mRNA inhibitory efficiency was 75.6% (P < 0.01). (B): Western blot analysis (7 days after infection) shows that in cells infected with the PscSI616 lentivirus, the protein inhibitory efficiency was 75.1% (P < 0.01) relative to the β-actin inhibitory efficiency. (C): mRNA efficiency in nude mice tumour tissue is 72.2% (PscSI616 vs. PscNC control; P < 0.01). (D): Protein inhibitory efficiency in nude mice tumour tissue is 74.1% (PscSI616 vs. PscNC control; P < 0.01).