Figure 4.
Cyclin B1 and Bcl-2 are downregulated after NSBP1 knockdown. (A): Cyclin B1 and Bcl-2 mRNA expression relative to GAPDH decreased by about 19.7% and 52.1% (PscSI616 vs. PscNC control) in vitro, respectively, and by 23.2% and 53.7%, respectively (PscSI616 vs. PscNC control), in nude mice tumour tissues (P < 0.01). (B): Western blot analysis showed that cyclin B1 and Bcl-2 expression relative to β-actin was decreased by about 20.0% and 46.8%, respectively, in vitro (P < 0.01). (C): Western blot analysis showed that cyclin B1 and Bcl-2 expression relative to β-actin was decreased by about 26.8% and 45.3%, respectively (PscSI616 vs. PscNC control), in vivo (P < 0.01).