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. 2010 Jul 26;12(5):744–752. doi: 10.1038/aja.2010.44

Figure 2.

Figure 2

Expression of tankyrase 1 in CSMCs from 8-month-old non-ED (erectile dysfunction) and 24-month-old ED rats. CSMCs were isolated and cultured primarily in complete medium using the enzyme digestion method. After the CSMCs adhered to the wall of the dishes and cell density reached 90% confluence, the CSMCs were used for protein isolation and subsequently used for Western blot analysis with anti-tankyrase 1 antibody. The relative expression was calculated by normalizing to β-actin. The expression of tankyrase 1 in 24-month-old ED rats' CSMCs was obviously less than that of 8-month-old rats' CSMCs (A). The relative expression was calculated by normalizing to β-actin (B). *P < 0.01, compared with CSMCs of 8-month-old rats.