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Colorimetric assay for the clonogenic survival after transfection. Absorbance at 590 nm was measured for the cell associated dye from stable clones obtained after transfection with PSP94 expression construct and empty vector (A) and for those obtained after CRISP-3 transfection and empty vector transfection (B). Optical density is plotted as mean ± SE. The experiment was repeated thrice and the graph is for one representative experiment.
