Table 1. Sequences of primers and TaqMan probes used in quantitative RT-PCR for 3β-HSD and Rpl19.
Gene | Primer/probe | Sequence (5′ to 3′) | Product length (bp) |
---|---|---|---|
3β-HSD | Forward | AGCAAAAAGATGGCCGAGAA | |
Reverse | GGCACAAGTATGCAATGTGCC | 80 | |
TaqMan | (FAM)TGGCAGCCAATGGGAGCATCCT(TAMRA) | ||
Rpl19 | Forward | CATGAGTATGCTTAGGCTACAGAAG | |
Reverse | GCGATTTCGTTGGTTTCATT | 96 | |
TaqMan | (FAM)CGCTGTGGTAAAAAGAAGGTGTGGTTG(TAMRA) |