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Merged fluorescent and DIC photos of bull spermatozoa labeled with Live/Dead Fixable Red Dead Cell Stain Kit (Invitrogen L-23102) and Alexa Fluor 488 PNA (L21409, green). Spermatozoa with intact plasma membrane and intact acrosome show no fluorescence, cells with disrupted plasma membrane show red tail and/or head and ruptured acrosomes are green. PNA, peanut agglutinin.
