Figure 4.

Simultaneous evaluation of viability and acrosome integrity (SYBR 14/PE-PNA/PI labeling). Color dot plot shows viable, acrosome-intact (lower left quadrant), viable, acrosome-ruptured (lower right quadrant), dead, acrosome intact (upper left quadrant) and dead, acrosome-ruptured spermatozoa (upper right quadrant). Green color indicates viable cells, red indicates dead ones, blue color indicates dying spermatozoa (evaluated as dead). PE, phycoerythrin; PI, propidium iodide; PNA, peanut agglutinin.