Table 1. Zona pellucida glycoproteins involved in sperm binding and induction of the acrosome reaction in mice and humans.
| Species | ZP protein | Function | Reference | |
|---|---|---|---|---|
| Binding to capacitated spermatozoa | Induction of acrosome reaction | |||
| Mice | Native ZP3 | Yes | Yes | 17, 47 |
| Recombinant ZP3 | Yes | Yes | 58 | |
| Humans | ZP1 | |||
| Baculovirus-expressed recombinant protein | Yes | Yes | 31 | |
| ZP3 | ||||
| Escherichia coli-expressed recombinant ZP3 | ND | Yes | 65 | |
| Yes | No | 53 | ||
| Baculovirus-expressed recombinant ZP3 | Yes | Yes | 52, 53 | |
| ND | Yes | 61 | ||
| ND | Yes | 32 | ||
| Mammalian-expressed recombinant ZP3 | ND | Yes | 62 | |
| Yes | Yes | 63 | ||
| ND | Yes | 64 | ||
| Native ZP3 | Yes | Yes | 55,60 | |
| ZP4 | ||||
| E. coli-expressed recombinant ZP4 | Yes | No | 53 | |
| Baculovirus-expressed recombinant ZP4 | Yes | Yes | 52,53 | |
| ND | Yes | 61 | ||
| ND | Yes | 32 | ||
| Native ZP4 | Yes | Yes | 55, 60 | |
Abbreviations: ND, not done; ZP, zona pellucida.