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. Author manuscript; available in PMC: 2014 Mar 13.
Published in final edited form as: J Am Chem Soc. 2013 Feb 27;135(10):4027–4039. doi: 10.1021/ja312457t

Figure 6.

Figure 6

Simulation of the EPR spectrum of the MnIIIMnIV intermediate. The spectrum was obtained by subtraction of a scaled spectrum of the MnII-loaded NrdF starting material from the spectrum of the RFQ mixture of 75 μM NrdF, 50 μM NrdI, and oxygenated buffer after 2 s aging time. Simulation of the spectrum was performed as described (section 2.9). Peaks 11-14 are labeled. Spectrometer settings: temperature: 10 K; microwave frequency = 9.38 GHz; power = 20 μW; modulation amplitude = 0.5 mT; modulation frequency = 100 kHz; sweep rate = 5.3 mT/s. The off-scale feature at ~330 mT is due to NrdIsq.