Table. HBoV1 DNA, other respiratory viruses, and HBoV antibodies in family members during 7 months, Finland*.
| Patient and no. days after hospital admission of index patient | Symptoms of acute infection | HBoV1 DNA in NPA, copies/mL | HBoV1 DNA in serum, copies/mL† | HBoV1 IgM‡ | HBoV IgG avidity | HBoV1 IgG‡ | HBoV2 IgG‡ | HBoV3 IgG‡§ | Other viruses detected in NPA by PCR |
|---|---|---|---|---|---|---|---|---|---|
| Index | |||||||||
| 1 | Yes | 1.6 × 1010 | NT | NT | NT | NT | NT | NT | None |
| 4 | Yes | 4.1 × 108 | 3,000 | 2.148 | 2.0 | 1.034 | 0 | 0 | HRV |
| 9 | Yes | 3.0 × 105 | 1,400 | 2.089 | 2.0 | 3.127 | 0 | 0.034 | HRV negative |
| 29 | Yes | 300 | 440 | 1.613 | 9.7 | 3.028 | 0.060 | 0 | HRV negative |
| 64 | No | NT | Negative | 0.154 | 17.1 | 3.426 | 0.050 | 0 | NT |
| 74 | No | 5,400 | NT | NT | NT | NT | NT | NT | None |
| 109 | Yes | 600 | Negative | 0.040 | 31.3 | 3.221 | 0 | 0 | PIV1 |
| 185 | ND | Negative | NT | NT | NT | NT | NT | NT | NT |
| 232 |
Yes |
Negative |
NT |
NT |
NT |
NT |
NT |
NT |
AdV |
| Twin brother | |||||||||
| 5 | Yes | 6,300 | NT | NT | NT | NT | NT | NT | HRV |
| 14 | ND | 7,800 | NT | NT | NT | NT | NT | NT | HRV |
| 29 | ND | 3,900 | NT | NT | NT | NT | NT | NT | HRV |
| 64 | No | NT | Negative | 0.022 | 26.9 | 3.305 | 0 | 0 | None |
| 74 | No | Negative | NT | NT | NT | NT | NT | NT | None |
| 109 | Yes | 99,000 | Negative | 0.011 | 38.2 | 3.242 | 0 | 0 | PIV1 |
| 232 |
Yes |
5,700 |
NT |
NT |
NT |
NT |
NT |
NT |
AdV |
| Mother | |||||||||
| 5 | No | Negative | NT | NT | NT | NT | NT | NT | HRV |
| 13 | ND | Negative | NT | 0.013 | 50.2 | 0 | 0.509 | 0.307 | HRV negative |
| 30 | No | Negative | NT | 0.019 | 45.0 | 0.001 | 0.542 | 0.360 | HRV negative |
| 64 | No | Negative | NT | 0.019 | 48.1 | 0.012 | 0.480 | 0.259 | NT |
| 109 |
No |
300 |
NT |
0.032 |
50.2 |
0 |
0.861 |
0.585 |
PIV1 |
| Grandmother | |||||||||
| 5 | Yes | 5,100 | NT | NT | NT | NT | NT | NT | None |
| 13 | ND | 22,000 | 960 | 0.011 | 58.0 | 0.395 | 0.542 | 0.016 | HRV negative |
| 64 | ND | NT | Negative | 0.012 | 25.4 | 2.731 | 1.470 | 0 | None |
| 74 | No | 3,600 | NT | NT | NT | NT | NT | NT | HRV negative |
| 109 | Yes | 300 | Negative | 0.023 | 33.5 | 2.674 | 1.834 | 0 | PIV1, HRV |
*HBoV1, human bocavirus 1; NPA, nasopharyngeal aspirate; NT, not tested; HRV, human rhinovirus; HRV negative, tested by monoplex PCR only; PIV1, parainfluenzavirus type 1; ND, no data available; AdV, adenovirus; none, negative by multiplex PCR. †All serum samples were negative for HBoV2–4 DNA (6). ‡HBoV antibodies after heterologous antigen competition are shown as absorbance values at 492 nm, cutoff 0.13 (7). §IgG avidity was calculated as the ratio of 2 IgG end-point titers (urea positive/urea negative) as described (8). Results obtained with unblocked enzyme immunoassay and confirmed with specific competition enzyme immunoassays.