Skip to main content
. 2013 Mar 18;15(3):375–381. doi: 10.1038/aja.2012.144

Figure 3.

Figure 3

The influence of GT1b and GM1 micelles on the onset of superoxide production by PMA-activated PMNs (5×106 ml−1) detected by EPR spectroscopy in the presence of the DEPMPO spin trap. The time-dependent superoxide anion generation by PMA-stimulated PMNs in the absence (filled circles) or presence (filled squares) of 100 µmol l−1 GM1 and GT1b (filled triangles). The data represent the mean±s.e.m. of five experiments. The typical experimental spectrum of the DEPMPO-OOH spin adduct is shown in the inset. *P<0.005 with GT1b compared to untreated cells. EPR, electron paramagnetic resonance; PMA, phorbol 12-myristate 13-acetate; PMN, polymorphonuclear neutrophils; DEPMPO, 5-diethoxyphosphoryl-5-methyl-1-pyrroline-N-oxide; DEPMPO-OOH, DEPMPO superoxide adduct.