Skip to main content
. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2013 Mar 28;33(6):1230–1237. doi: 10.1161/ATVBAHA.113.301436

Figure 1. Cocaine induces VWF release from HUVEC, BMVEC, and CAEC.

Figure 1

Figure 1

Cultured endothelial cells at passage 2–4 were incubated with the indicated agonists or serum-free medium (untreated) at 37°C. VWF abundance was determined in cell supernatants by dot blot analysis using an HRP-conjugated detection antibody and expressed in milliunits (mU) calibrated to a standard curve of normal human reference plasma. A) VWF release following incubation with cocaine at the indicated concentrations for each of the three endothelial cell types. VWF release following incubation with cocaine metabolites was also assessed from HUVEC (B), BMVEC (C), and CAEC (D) as compared to histamine 50μM or dDAVP 4μg.ml. The results are expressed as means +/− SEM. * Tukey’s HSD test, as compared to untreated cells (p < 0.05). ~ Tukey’s HSD test, not different than histamine treated cells (p > 0.05).