Skip to main content
. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2013 Mar 28;33(6):1230–1237. doi: 10.1161/ATVBAHA.113.301436

Figure 4. Cocaine, benzoylecgonine, and cocaethylene induce secretion of ULVWF.

Figure 4

Endothelial cells exposed to the indicated agonsists (H = histamine 100μM, C = cocaine1μg/ml, BE = benzoyecgonine 10μg/ml, CE = cocaethylene 10μg/ml). Supernatants were then subjected to non-reducing gel electrophoresis, and VWF multimer patterns were assessed by Western blot. Normal pooled human plasma (NPP) was used as a control with 0.0125 μl loaded (left lane) and 0.025 μl loaded (right lane). For each panel, lanes are derived from a single gel. The dashed line represents the same band number (14) counted from the bottom applied to each cell type. Higher molecular weight forms (ULVWF) are present above the dotted line.