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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: J Mol Cell Cardiol. 2013 May 30;62:144–152. doi: 10.1016/j.yjmcc.2013.05.014

Figure 6. Representative immunoblots with densitometric analyses for proteins involved in regulation of fatty acid oxidation.

Figure 6

(A) The PDK4 expression levels shown normalized to mean CON-S intensity relative to GAPDH were increased in ECMO groups with systemic (S) and intracoronary infusion (IC) respectively compared with CON. No changes in protein expression between groups for PDK2 (B), CD36 (C) or Glut4 (D) were significant. Furthermore, no significant difference occurred between groups for phosphorylation state (blot intensity phosphorylation/total) for AMPK (E)) and ACC (F). Phosphorylation of each protein was detected on the same gel of each protein following re-probing of membranes. GAPDH was used as loading control and varied similarly to total protein by Ponceau S staining. All data are representative of at least three independent experiments. Values are means ± SE; n = 5-6 per group. *: P < 0.05 vs CON.