| CaCl2 (2.0 M) | 1.15 mL |
| ΔΦ vector plasmid (containing the desired transgene) | 272 μg |
| pCiGSΔΨ | 272 μg |
| pCiPS | 34.5 μg |
| pCiES | 16.9 μg |
| Sterile H2O | 8.05 mL |
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