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. 2013 Aug 9;8(8):e71479. doi: 10.1371/journal.pone.0071479

Figure 1. Immunopositive staining of CA1 pyramidal cell nuclei (NeuN) and inactive GSK3β (cytoplasm).

Figure 1

To visualize pyramidal cells for cell counting an antibody against NeuN, a nuclear marker was used along with SG, a dark grey stain. Inactive GSK3β (GSK3βps9) in the cytoplasm of pyramidal cells in the CA1 was visualised using an antibody against GSK3βps9 along with DAB, a brown stain. A double immunopositive staining was performed to count the number of CA1 pyramidal cells that contained inactive GSK3β in the cytoplasm using antibodies against NeuN and GSK3βps9 with SG and DAB respectively. Scale Bar = 40 µm.