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. 2013 Aug 9;8(8):e73366. doi: 10.1371/journal.pone.0073366

Figure 3. Effect of ST8SiaII inhibition on cell surface polySia expression.

Figure 3

Upper: Flow cytometry analysis of effect of 24h exposure to increasing CMP concentrations on polySia expression in IMR-32 cells (NCAM +; ST8SiaII +; polySia +). Lower: Relative mean fluorescence intensities as a result of CMP treatment. Cells were labelled with EndoNA2-eGFP and analysed by flow cytometry. Fluorescence is expressed as a mean percentage of that observed with the control (untreated cells) ± SD of three independent experiments (* P < 0.01, ** P < 0.001). CMP causes a significant and concentration-dependent reduction in polySia expression in IMR-32 cells.