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His-tag cyclophilin vector (CV) and nonvector (CNV) isoforms were expressed in vitro in E. coli and co-immunoprecipitated with CYDV-RPV or PLRV. Co-immunoprecipitated proteins were detected with anti-his antibodies. First lane shows the synthesized cyclophilin protein. Interactions were notable between both isoforms and CYDV-RPV but not between PLRV and the vector isoform.
