Table 1. Serotype specific primers used in this study.
| Serotypes | Sequences (5′–3′)d | Targeted Genes | GenBank accession no. | Multiplex PCR assaye | PCR product size (bp) |
|---|---|---|---|---|---|
| 1 & 14a | Forward: 450-TCTTATAACAGGCGTCAAAACA-471 | cps1I | JX986790 | 1st | 153 |
| Reverse: 602-ATCGGTATAAAAGCAAGACACA-581 | JX986804 | ||||
| 2 & 1/2a | Forward: 544-TTCGTATTAACTTACTTGGCGT-565 | cps2I | KC537364 | 1st | 363 |
| Reverse:906-TAAATCCCCATATGCCAAATCC-885 | KC537384 | ||||
| 3a | Forward: 442-ACATCCATTGCAGGAGTAGT-461 | cps3L | KC537365 | 1st | 210 |
| Reverse:651-TGCAGTTCCAAAATTCTTCGT-631 | |||||
| 4a | Forward: 399-TGATATTGGCTATCTTTTGGGG-420 | cps4K | KC537366 | 1st | 542 |
| Reverse: 940-TTCCCCCTTCAAATAAACTCTG-919 | |||||
| 5a | Forward: 583-AGGTATGTCTTCTTATTCGCAG-604 | cps5L | KC537367 | 1st | 428 |
| Reverse: 1010-ATAATCCCTCCTGATACTAGGC-989 | |||||
| 6a | Forward: 141-TGGTGTCTTTCTACCTGCAA-160 | cps6I | KC537368 | 1st | 705 |
| Reverse:845-TCACCAAGATACGTGAACCA-826 | |||||
| 7a | Forward: 364-AAAATTCGTTCCATTGTAGGTG-385 | cps7L | KC537369 | 1st | 609 |
| Reverse: 972-TGAAGTTGAAGCTGGTGATAAA-951 | |||||
| 8a | Forward: 130-ATCGCTTCAAATAAGGTAGGAG-151 | cps8K | JX986797 | 1st | 268 |
| Reverse: 397-TGTAGGCCGTAATATCAACAAA-376 | |||||
| 9a | Forward:2-TGAAAGTAGGTATATCTCAGCA-23 | cps9J | KC537370 | 1st | 809 |
| Reverse: 810-AAAGAATTGAATCCCACCTGAG-789 | |||||
| 10a | Forward:25-CTATCACTACCACGGAATGC-44 | cps10M | JX986799 | 1st | 303 |
| Reverse:327-TAACCGTCCGTCTAGAATGT-308 | |||||
| 11a | Forward: 46-ATTGTTACGATTTGGGCGAT-65 | cps11N | KC537371 | 2nd | 512 |
| Reverse:557-GAACCCCATGTAGTTATGGC-538 | |||||
| 12a | Forward:1295- CATGGGAACTGTACAGGATAAG-1316 | cps12J | KC537372 | 2nd | 171 |
| Reverse: 1465-CCACCTTACTACCTGTTTTACC-1444 | |||||
| 13a | Forward: 30-GCTTGTAGCGAATTTTGGTATT-51 | cps13L | JX961643 | 2nd | 741 |
| Reverse:770-CCATTAGATGTATTTGCTCCCA-749 | |||||
| 15a | Forward: 565-ACCTACTCAAGAACATCCTTTC-586 | cps15K | JX961644 | 2nd | 458 |
| Reverse: 1022-GTAACTAAAACAGCAAACGTCA-1001 | |||||
| 16a | Forward: 551-ATCAACAAACATTTTCGAGGAC-572 | cps16I | KC537373 | 2nd | 223 |
| Reverse: 773-GCTGAATAATAGATTCGTCCTGT-751 | |||||
| 17a | Forward: 37-TTGCCGTATAAGGTCTTAGTTG-58 | cps17O | KC537374 | 2nd | 380 |
| Reverse: 416-ATCTGACGGTAAATGTTCTCTG-395 | |||||
| 18a | Forward: 689-ATAGGCTGTACTTTGATAACCG-710 | cps18N | KC537375 | 2nd | 310 |
| Reverse:998-AGCCTATCGCTCAAAAACTTAT-977 | |||||
| 19a | Forward:589-ATTATTATAGGGCAAAGCAGGG-610 | cps19L | KC537376 | 2nd | 674 |
| Reverse:1262-ATCGTACACAACAAAACGATTC-1241 | |||||
| 20a | Forward: 236-TAATCGTTGCCTTTGAGCAT-255 | cps20I | KC537377 | 2nd | 938 |
| Reverse: 1173-CGCTATATAAGGAAACCTCGG-1153 | |||||
| 21a | Forward: 218-TGGCAGACTTCTTTTCTCAC-237 | cps21P | KC537378 | 2nd | 858 |
| Reverse: 1075-CCTGTAGCGCCTCATAAAAC-1056 | |||||
| 22a | Forward: 183-AGGATCGGTAAGTTTAGGTACA-204 | cps22K | KC537379 | 3rd | 158 |
| Reverse:340-ATGCAGTAAAACACGAAAACAA-319 | |||||
| 23a | Forward: 250-TATTATAGTCCGATGCAAGCAG-271 | cps23J | JX986802 | 3rd | 461 |
| Reverse:710-ATGAGAACGAAACGGAATAGTT-689 | |||||
| 24a | Forward: 736-GATAGCAATGTAATCCAATCGC-757 | cps24M | KC537380 | 3rd | 204 |
| Reverse:939-GTAGGTTCCCCTAGTAAGAAGT-918 | |||||
| 25a | Forward: 477-ATTGAGTCCTTTTACTGGTAGC-498 | cps25M | JX986803 | 3rd | 390 |
| Reverse:866-TACTGAGCTACATAATCCCACA-845 | |||||
| 26a | Forward:663-CAAAATTCCTGGATTAACGCTT-684 | cps26P | KC537381 | 3rd | 315 |
| Reverse:977-CGATCTGAGGACTTATCAAGAA-956 | |||||
| 27a | Forward: 354-GTGGTTTTGGAGGATATTTTCG-375 | cps27K | JX961652 | 3rd | 530 |
| Reverse:883-ATTGAGATAAACTACTCCGTGC-862 | |||||
| 28a | Forward: 38-GGGCACTTGTTTTACTTCCT-57 | cps28L | JX961653 | 3rd | 896 |
| Reverse:933-GCCAAGTAATACCCTACCTG-914 | |||||
| 29a | Forward: 314-AAAGTGCCTATTCTGGGATTTT-335 | cps29L | JX961654 | 3rd (4th) | 263 |
| Reverse:576-TAAAGGCAACTTCCACATTGTA-555 | |||||
| 30a | Forward: 581-TTGGGCTTGTAAATAGTGAGAG-602 | cps30I | KC537382 | 3rd | 625 |
| Reverse:1205-CGATTAGATAAGCGCATTTGTT-1184 | |||||
| 31a | Forward: 19-CATATGTTTTCGTGGGGAGT-38 | cps31L | JX961656 | 3rd | 1006 |
| Reverse:1024-GTGATGAAAACATCGTTGGTAG-1003 | |||||
| 33a | Forward: 353-GAGTTGCGACCTATTATTCTCA-374 | cps33K | KC537383 | 3rd | 731 |
| Reverse:1083-GAATTGAACAACGACTGCAATA-1062 | |||||
| 21b | Forward: 13-TTGATAACAGGAGCAAACTCAT-34 | cps21H | KC537385 | 4th | 455 |
| Reverse: 467-TTACCATAAATCATCGGTGGTC-446 | |||||
| 21b | Forward: 78-AGTAGAAAGAGGGTACAAGGTT-99 | cps21I | KC537385 | 4th | 311 |
| Reverse: 388-CAGGTATGTTCCGTTTAGAACT-367 | |||||
| Allc | Forward: 1180-GAAAATATGAAGAGCCATGTCG-1201 | thrA | CP000837 | All 4 | 120 |
| Reverse: 1299-GACAACGAACATAACAGAAACTTC-1276 |
a Primers used in the multiplex PCR reactions.
b Primers used for screening the distribution of the cpsH and cpsI among 19 isolates which were positive for serotype 29 by mPCR.
c The thrA gene refer to S . suis GZ1 (GenBank NO. CP000837).
d The numbers flanking the oligonucleotide primers represent the positions in the target genes.
e Primers included in one or more of the four mPCR assays.