Figure 3. Furazolidone induces apoptosis of AML cells.

The apoptosis was measured by Annexin V-PE/7-AAD kit using FACS in Kasumi-1, NB4, MV4-11 and MolM13 in the presence of DMSO control or the predetermined IC50 value of FZD for 72 hours.There are reports that FZD can inhibit cell proliferation and provokes apoptosis by inducing S-phase arrest in human hepatoma G2 cells [14]. We therefore tested whether FZD could also cause cell-cycle arrest in AML cells. We treated AML cells with FZD at the predetermined IC50 value and analyzed by flow cytometry. We showed that this compound did not modulate cell-cycle distribution (Figure S1), suggesting that it prolonged cell-doubling time rather than inducing cell-cycle arrest.