Table 2. Thermodynamic parameters and association constant of HSA-HA obtained by isothermal titration calorimetry.

	T (°C)	Kb (M−1)	ΔH° a	TΔS° a	ΔG° a
High affinity site	25	(2.75±0.51)×104	−4.41±0.46	1.90±0.30	−6.05±0.10
	30	(1.95±0.05)×104	−5.18±0.07	0.76±0.06	−5.94±0.01
	37	(1.54±0.06)×104	−6.14±0.15	−0.20±0.01	−5.93±0.02
Low affinity site	25	(2.80±0.49)×103	−7.58±0.96	−2.88±0.40	−4.69±0.10
	30	(1.38±0.03)×103	−9.74±0.34	−5.39±0.35	−4.35±0.02
	37	(1.29±0.07)×103	−11.11±0.69	−6.69±0.36	−4.41±0.03

^aexpressed in kcal mol^−1.