Table 2. Thermodynamic parameters and association constant of HSA-HA obtained by isothermal titration calorimetry.
T (°C) | Kb (M−1) | ΔH° a | TΔS° a | ΔG° a | |
High affinity site | 25 | (2.75±0.51)×104 | −4.41±0.46 | 1.90±0.30 | −6.05±0.10 |
30 | (1.95±0.05)×104 | −5.18±0.07 | 0.76±0.06 | −5.94±0.01 | |
37 | (1.54±0.06)×104 | −6.14±0.15 | −0.20±0.01 | −5.93±0.02 | |
Low affinity site | 25 | (2.80±0.49)×103 | −7.58±0.96 | −2.88±0.40 | −4.69±0.10 |
30 | (1.38±0.03)×103 | −9.74±0.34 | −5.39±0.35 | −4.35±0.02 | |
37 | (1.29±0.07)×103 | −11.11±0.69 | −6.69±0.36 | −4.41±0.03 |
expressed in kcal mol−1.